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Cytiva HiPrep™ IMAC FF 16/10 Column
For optimizing purification of histidine-tagged proteins at laboratory scale
Brand: Cytiva 28-9365-52
This item is not returnable.
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Description
IMAC Sepharose™ 6 Fast Flow is prepacked in 20mL HiPrep columns for greater scale up. The medium consists of 90μm beads of highly crosslinked agarose, to which a chelating group has been covalently coupled. This group may be charged with suitable metal ions, specific for your target protein.The bead size enables high flow properties; low metal ion leakage retains activity of the purified protein.
Specifications:
IMAC Sepharose 6 Fast Flow
- Matrix: highly crosslinked agarose, 6%
- Average particle size: 90μm
- Binding capacity (protein/ion dependent): approx. 40mg histidine-tagged protein/mL medium when charged with Ni2+
- Compatibility: stable in all commonly used buffers
- Chemical stability (uncharged medium) one week, 40°C: 0.01 M HCl, 0.1 M NaOH; 12 hr.: 1M NaOH, 70% acetic acid; 1 hr. tested 2% SDS; 30 min. tested 30% 2-propanol
- Stability (pH, w/ uncharged medium w/ metal ion): 3 to 12 (long-term), 2 to 14 (short-term)
- Storage/Temperature: 20% ethanol; 4° to 30°C
HiPrep Prepacked Columns
- Volume: 20mL
- Dimensions: 1.6 x 10cm
- Max. pressure during operation: 1.5bar (0.15MPa, 22psi)
- Hardware pressure limit: 5bar (0.5MPa, 73psi)
Specifications
| 0.01M HCl, 1M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-propanol | |
| 20mL | |
| 16mm | |
| 3.93 in. | |
| <10mL/min. | |
| 1.5 bar | |
| 45 to 165μm | |
| 4° to 30°C | |
| IMAC and subsequent purification of Polyhistidine tagged Proteins |
| HiTrap IMAC Sepharose 6 FF column | |
| 0.62 in. | |
| 1 x 20mL | |
| 100mm | |
| Polypropylene | |
| 3 to 12 | |
| 6% Cross-linked Agarose | |
| Ion Exchange Column |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- HiPrep IMAC FF 16/10
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol