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Gibco™ RPMI 1640 Medium, no folic acid
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
Brand: Gibco™ 27016021
Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Folic Acid
The complete formulation is available.
Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco RPMI 1640 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Leukemic Cells | |
| 1 X | |
| RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) | |
| Sterile-filtered | |
| No HEPES, No Sodium Pyruvate, No Folic Acid | |
| Gibco™ | |
| Room Temperature |
| HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| Glutamine, Phenol Red | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- RPMI 1640 Medium, no folic acid
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P240-Ground/bond container and receiving equipment.
- P243-Take precautionary measures against static discharge.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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