missing translation for 'onlineSavingsMsg'
Learn More

Macherey-Nagel™ Genomic DNA isolation, Wash Buffer BW

Brand:  Macherey-Nagel™ 740922


Product Code. 10756912

  • 40.95 € / 100mL

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

NucleoSpin™ Tissue is designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples and clinical samples.
Up to 35μg of high purity genomic DNA can be prepared (typical yields from tissue or cells: 15 to 25μg). The obtained DNA can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. With the NucleoSpin™ Tissue method, lysis is achieved by incubation of the samples in a solution containing SDS and proteinase K at 56°C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Tissue columns are created by addition of large amounts of chaotropic ions (buffer B3) plus ethanol to the lysate. The binding process is reversible and specific for nucleic acids. Contaminations are removed by efficient washing. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and is ready to use for subsequent reactions.Kit components: NucleoSpin™ Tissue columns, 2mL collecting tubes, buffers, proteinase K. For further information on this product please contact Customer Services.
  • Silica membrane technology
  • Sample size: up to 25mg to tissue or 107 cells
  • Yield: up to 35μg genomic DNA
  • Elution volume: 60μL to 100μL
  • Binding capacity: 60μg
  • Preparation time: 20min/4 to 6 preps
  • Format: mini spin columns
  • Spin columns can be closed – no cross-contamination
  • No use of organic solvents
  • Highly pure nucleic acids suitable for all common downstream applications
Total DNA extraction possible from: Tissue (e.g. mouse tails); Cells (e.g. bacteria); Clinical samples (stool, urine, biopsy samples); Forensic samples (dried blood spots, hair, buccal swabs, cigarette filters).
Specifications

Specifications

100mL
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash with water thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.

Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride, 2-propanol

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.