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Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Brand: Cytiva 27-0846-01

Product Code. 10218854

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Description

Supplied as lyophilized powder
Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

Specifications

Buffer	Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄ pH 7.3
Product Type	Thrombin Protease Fusion Protein
Quantity	500U
For Use With (Application)	Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours

Product Suggestions Safety and Handling

Safety and Handling

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Hazard Category

Hazard Statement

Precautionary Statement

P280-Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

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