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Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

761.00 € - 8900.00 €

Specifications

Binding Capacity >10 mg/mL
Description Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Flow Rate <450 cm/hr.
Kit Contents 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Matrix Highly cross-linked 4% agarose
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
11595015
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Cytiva
17-5132-02
100 mL
2264.00 €
100mL
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11585015
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Cytiva
17-5132-01
25 mL
761.00 €
25mL
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11505025
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Cytiva
17-5132-03
500 mL
8900.00 €
500mL
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Description

Description

  • Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
  • Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
  • Binding capacity and high flow properties allow scale-up
  • Mild elution conditions preserve protein antigenicity and function
  • Stable in all commonly used aqueous buffers
  • pH stability: 3 to 12

Specifications

Specifications

>10 mg/mL
<450 cm/hr.
Highly cross-linked 4% agarose
4% Highly Cross-linked Agarose
base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Chromatography Media
Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
3 to 12
4°C to 30°C, 20% Ethanol
90 μm
Glutathione and 10-carbon linker arm
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