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Cytiva Ficoll PM400
High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. Cytiva™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
240.00 € - 7184.00 €
Specifications
| Content And Storage | 4–30°C |
|---|---|
| For Use With (Application) | Cell Isolation |
| Viscosity | 5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v) |
| Endotoxin Level | 0.12 EU/mg |
| Color | White |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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11580724
|
Cytiva
17-0300-10 |
100 g |
240.00 €
100g |
In Stock
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11590724
|
Cytiva
17-0300-50 |
500 g |
933.00 €
500g |
In Stock
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11570724
|
Cytiva
17-0300-05 |
5 kg |
7184.00 €
5kg |
In Stock
Log in to see stock. |
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Description
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
- Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
- Has better osmotic properties than sucrose
- Preserves functional and morphological integrity
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
- Does not penetrate biological membranes
- Serves as the raw material for preparation of Ficoll-Paque gradients
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
- Supplied as dry powders
- Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
- Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
- Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity
Specifications
| 4–30°C | |
| 5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v) | |
| White | |
| 1.2 g/mL |
| Cell Isolation | |
| 0.12 EU/mg | |
| Centrifugation Media | |
| Powder |
Safety and Handling
missing translation for 'shelfLife' : 5 years
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