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Fisher BioReagents™ SurePrep™ Soil DNA Isolation Kit
Provides a convenient and rapid method for the isolation and purification of total DNA from microorganisms in soil and sediment samples, such as bacteria, fungi, and algae
Brand: Fisher BioReagents™ BP2815-50
Includes: Lysis additive (6mL); Binding solution (6mL); Lysis solution (45mL); Wash solution I (30mL); Wash solution II (18mL); Elution buffer (6mL); Spin columns (50); Collection tubes (50); Beads (30g); Screw cap tubes (50); Elution tubes, 1.7 mL (50)
Description
- All types of soil samples can be processed, including common soil samples and difficult soil samples with a high humic acid content such as compost and manure
- Optimized protocol to remove excess water can be used to evaluate sediment samples with high water content, such as those taken from ponds or lakes
- Kit includes specialized solutions and bead tubes which remove all traces of humic acids through chemical and physical separation and lysis procedures
- A simple and rapid spin column procedure using a proprietary resin to further purify the DNA
- All humic acid substances and PCR inhibitors are removed before the DNA isolation procedure
- Fast and easy procedure can be completed in 30 minutes
- Protocol does not require use of hazardous chemicals such as phenol and chloroform or require an additional time-consuming step of ethanol precipitation to recover DNA during the purification process
- Purified DNA can be used for numerous downstream applications for the quantification and analysis of DNA, including PCR and QPCR
Specifications
| Rapid method for the isolation and purification of total DNA from microorganisms in soil and sediment samples, such as bacteria, fungi and algae | |
| 6mL Lysis additive, 6mL binding solution, 45mL Lysis solution, 30mL wash solution I, 18mL wash solution II, 6mL Elution Buffer, 50 spin columns, 50 collection tubes, 30g beads, 50 screw cap tubes, 50 1.7mL elution tubes | |
| 50 Preps |