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Thermo Scientific™ FastDigest DpnI
Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).
41.33 € - 77.00 €
Specifications
| Methylation Sensitivity | Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive |
|---|---|
| Enzyme | Dpn I |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Sensitive to Heat Inactivation | Yes |
| Optimal Reaction Temperature | 37°C |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10809410
|
Thermo Scientific™
FD1703 |
50 μL (50 Reactions) |
41.33 €
50 reactions |
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|
10819410
|
Thermo Scientific™
FD1704 |
100 μL (100 Reactions) |
77.00 €
100 reactions |
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Description
Lambda DNA digested with DpnI, 1.4% agarose, 22 cleavage sites
FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Highlights
- 100% activity of all FastDigest enzymes in the universal buffer
- 100% buffer compatibility with downstream applications
- Complete digestion in 5-15 minutes
- Direct loading on gels
- No star activity
- 176 FastDigest enzymes available
Specifications
| Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| 10X FastDigest Buffer/FastDigest Green Buffer | |
| 37°C | |
| Restriction Enzyme | |
| FastDigest™ | |
| 80°C |
| Dpn I | |
| Yes | |
| No | |
| Traditional Cloning | |
| Blunt |
For Research Use Only. Not for use in diagnostic procedures.