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Gibco™ Expi293F™ Cells
Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System
1186.00 € - 4995.00 €
Specifications
Cell Line | Expi293F |
---|---|
Cell Type | Kidney (Embryonic) |
Culture Type | Suspension Cell Culture |
Form | Cryopreserved |
Species | Human |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
13305554
|
Gibco™
A14527 |
1 mL |
1186.00 €
Each |
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13479756
|
Gibco™
A14528 |
6 x 1 mL |
4995.00 €
6mL |
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Description
- Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System
- They are maintained in suspension culture and will grow to high density in Expi293™ Expression Medium
- Expi293F™ cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression
- Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293™ Expression Medium and 10% DMSO
- The cells should be thawed directly into Expi293™ Expression Medium
- The six-vial multipack eliminates the need to expand and rebank the cells in the lab
- Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator
- However, cultures are scalable from volumes of 10 L in bioreactors
- Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed
- For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection
- There is no need for culture medium supplementation or batch feeding during expression
Specifications
Expi293F | |
Suspension Cell Culture | |
Human | |
1 x 107 |
Kidney (Embryonic) | |
Cryopreserved | |
Expi293™ Expression Medium | |
Expi293™ |
For Research Use Only. Not for use in diagnostic procedures.