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IBA Lifesciences CD3+ CD45RO+ Tcm Fab Streptamer™ Isolation Kit MB
CD3+ CD45RO+ Tcm Fab Streptamer Isolation Kit MB is for reversible cell isolation delivering label-free target cells.
Brand: IBA Lifesciences 6-8000-217
This item is not returnable.
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Description
- Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 4 vials for 5 x 108 cells each
- CD3 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 109 cells
- CD45RO Fab-Strep, human for isolation of T-cells; 3 vials for 2 x 108 cells
- Biotin stock solution for Streptamer™ technology; 3 x 1 mL
- Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 2 x 50 mL
- Isolation of human CD3+ CD45RO+ central memory T cells is performed by serial double positive selection.
- In a first step PBMCs are labeled with magnetic CD3 Fab Streptamers™.
- Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
- This CD3 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
- The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
- Biotin is removed by washing.The pure and label-free CD3 positive cells are subjected to a second positive selection with magnetic CD45RO Fab Streptamers™ following the same procedure.
- The resulting cell preparation is highly enriched with CD3+ CD45RO+ central memory T cells with a purity of more than 90%.
- No columns are needed.
Specifications
| PBMCs | |
| Magnetic Microspheres Coated with Strep-Tactin | |
| 1.6 x 109 |