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Proteintech™ AuthentiKine™ Human IFN-gamma ELISA Kit
Solid phase sandwich ELISA used to detect and quantify protein levels of endogenous IFN-gamma
Brand: Proteintech™ KE00146-96T
This item is not returnable.
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Description
- Recognizes human IFN-gamma
- IFNG is a soluble cytokine that is the only member of type II class of interferons
- Associated with antiviral, immunoregulatory and anti-tumor properties
- Potent activator of macrophages
Process:
- Antibody specific for IFN-gamma pre-coated onto microwells
- IFN-gamma protein in samples captured by the coated antibody after incubation
- After extensive washing, another antibody specific for IFN-gamma is added to detect captured IFN-gamma protein
- For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent
- Solution containing sulfuric acid used to stop color development and color intensity
- Proportional to quantity of bound protein, measurable at 450 nm with correct wavelength set at 630 nm
Specifications
BC070256 | |
0.4 pg/mL | |
Sandwich ELISA, Double Antibody | |
Plasma, Serum | |
Human | |
Human | |
<10% | |
Blue ice | |
4.5 hr. |
7.8 to 500 pg/mL | |
ISO 13485 | |
Coated ELISA Kit | |
DAB | |
3458 | |
Solid | |
<10% | |
-20°C to +4°C | |
P01579 |