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V5 Tag Monoclonal Antibody, HRP, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen R96125
This item is not returnable.
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Description
NOTE: R961-25 was previously sold under product #46-0708. When re-ordering, please use #R961-25. R961-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. This antibody is functionally tested against 500 ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 10 minute exposure. This antibody has also been tested in Western blot against 50 ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM). Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.
V5 Tag Monoclonal antibody specifically detects V5 Tag in Tag samples. It is validated for ELISA, Western Blot.
Specifications
| V5 Tag | |
| Monoclonal | |
| PBS with no preservative | |
| Mouse | |
| purified | |
| RUO | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid | |
| Tag |
| ELISA, Western Blot | |
| HRP | |
| simian virus 5 antibody; SPV5gp2; sv5; V5; v-5; V5 Epitope Tag | |
| V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-. | |
| 50 μL | |
| Primary | |
| Antibody | |
| IgG2a |