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Invitrogen™ TRIzol™ Max™ Bacterial RNA Isolation Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria.
Brand: Invitrogen™ 16096020
501.50 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Description
The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol™ Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Key Features of the TRIzol™ Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol™ Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol™ Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol™ Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Specifications
• 20 mL Max Bacterial Enhancement Reagent; room temperature • 100 mL TRIzol Reagent; room temperature |
|
1 hr. | |
Bacteria (Gram +/-) | |
Not High-throughput Compatible (Manual) | |
100 Preps | |
Cells: ≤108 Bacterial culture: 1.5 mL |
Organic Extraction | |
50 μL | |
Total RNA | |
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling | |
Room Temperature | |
Gram-negative bacteria: >20 μg Gram-positive bacteria: ≤3 μg |
For Research Use Only. Not for use in diagnostic procedures.