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Invitrogen™ PureLink™ RNA Mini Kit
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment.
Brand: Invitrogen™ 12183025
1099.50 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Description
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 μg). This means that users can process both small and large sample sizes with the same RNA isolation kit.
- Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 106 to 108 tissue culture cells with one spin column in 20 minutes
- Isolate more RNA than competitor mini spin columns: up to 1000 μg of total RNA from one column
- Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)
- Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set (Cat. No. 12185010) for easy removal of residual DNA
Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment
The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction (see Figure 1). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination. The extra-large binding capacity enables one kit to handle most RNA isolation needs in a quick 20 minute protocol without the need for special sample processing instruments.
Easy, optional, on-column DNase treatment for sensitive applications
The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total RNA while removing the majority of genomic DNA. In general, most applications requiring RNA from animal tissue or mammalian cell lines do not require additional DNase treatment. However, some applications such as gene expression analysis by qRT-PCR without intron-spanning primers or working with samples from organisms with very small or no introns may require more complete removal of residual contaminating DNA. The PureLink DNase Set (12185010) allows for convenient on-column digestion of DNA during the RNA isolation protocol. Treating with DNase while 'on-column' is easier and allows higher RNA recovery than treating with DNase after the RNA has been isolated. The PureLink DNase Set can also be used to remove residual DNA from RNA that has been previously purified. Both the 'on-column' and post-RNA purification workflows are options available with the PureLink DNase Set.
Simplified, non-toxic RNA purification
The PureLink RNA Mini Kit utilizes non-toxic guanidine-isothiocyanate lysis buffer to protect the RNA during the isolation steps. Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration allow for a safe and easy procedure that can typically be completed in less than 20 minutes without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation.
The PureLink RNA Mini Kit is recommended for use with the Homogenizer (Cat. No. 12183026), designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.
Specifications
• 500 mL Lysis Buffer; store at room temperature • 250 mL Wash Buffer I; room temperature • 75 mL Wash Buffer II; room temperature • 75 mL RNase-free Water; room temperature • 250 PureLink Spin Cartridges with Collection Tubes; room temperature • 250 Collection Tubes; room temperature • 250 Recovery Tubes; room temperature |
|
20 min. (After sample homogenization) | |
Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions | |
Not High-throughput Compatible (Manual) | |
RNA Mini Kit | |
Mini | |
Bacteria: ≤109 cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108 cells, Enzymatic reactions: ≤1.2 mL |
Silica Spin Column | |
30–300 μL | |
Total RNA | |
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
250 Preps | |
Room Temperature | |
Up to 350 μg |
Safety and Handling
- RNA Mini Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 1 C
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Thiocyanic acid
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For Research Use Only. Not for use in diagnostic procedures.