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Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
BIOPHARMA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

287.64 € - 1489.01 €

Specifications

Pore Size 100 Å
USP Type L1
Stationary Phase C18
Phase Reversed Phase
View More Specs

Products 39
Product Code Brand Length (Metric) Diameter (Metric) Particle Size Type Price Quantity & Availability  
Product Code Brand Length (Metric) Diameter (Metric) Particle Size Type Price Quantity & Availability  
11342263
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Thermo Scientific™
164567
50 mm 0.075 mm 3 μm Analytical Column, NanoViper This item is currently unavailable or has been discontinued.
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N/A
11352263
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Thermo Scientific™
164568
150 mm 0.075 mm 3 μm Analytical Column
1189.41 €
Each
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11362263
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Thermo Scientific™
164569
250 mm 0.075 mm 3 μm Analytical Column
1298.71 €
Each
Estimated Shipment: 07-01-2025
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11372263
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Thermo Scientific™
164570
500 mm 0.075 mm 3 μm Analytical Column
1489.01 €
Each
Estimated Shipment: 07-01-2025
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11302273
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Thermo Scientific™
164572
150 mm 1 mm 3 μm Analytical Column
889.81 €
Each
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11312113
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Thermo Scientific™
160434
5 mm 1 mm 5 μm Trap Cartridge
559.34 €
Each
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11362113
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Thermo Scientific™
160454
5 mm 0.3 mm 5 μm Trap Cartridge This item is currently unavailable or has been discontinued.
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N/A
11322113
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Thermo Scientific™
160438
15 mm 1 mm 5 μm Trap Cartridge This item is currently unavailable or has been discontinued.
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N/A
11382243
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Thermo Scientific™
164197
150 mm 0.1 mm 5 μm Trap Column This item is currently unavailable or has been discontinued.
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N/A
11332253
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Thermo Scientific™
164213
150 mm 0.2 mm 5 μm Trap Column
328.42 €
Pack of 2
Estimated Shipment: 07-01-2025
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11392243
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Thermo Scientific™
164199
150 mm 0.1 mm 5 μm Trap Column
402.48 €
Pack of 2
In Stock
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11352103
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Thermo Scientific™
160323
150 mm 0.075 mm 5 μm Analytical Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
11362103
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Thermo Scientific™
160326
250 mm 0.075 mm 5 μm Analytical Column This item is currently unavailable or has been discontinued.
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N/A
11352023
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Thermo Scientific™
164562
150 mm 0.05 mm 2 μm Analytical Column, NanoViper This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
11372013
BIOPHARMA
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Thermo Scientific™
164537
150 mm 0.3 mm 2 μm Analytical Column
880.81 €
Each
In Stock
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11392253
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Thermo Scientific™
164563
50 mm 0.75 mm 2 μm Analytical Column, NanoViper This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
11382013
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Thermo Scientific™
164540
500 mm 0.75 mm 2 μm Analytical Column This item is currently unavailable or has been discontinued.
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N/A
11342013
BIOPHARMA
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Thermo Scientific™
164534
150 mm 0.075 mm 2 μm Analytical Column
1150.73 €
Each
In Stock
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Edge
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11342023
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Thermo Scientific™
164561
50 mm 0.05 mm 2 μm Analytical Column, NanoViper This item is currently unavailable or has been discontinued.
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N/A
11332023
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Thermo Scientific™
164560
50 mm 0.3 mm 2 μm Analytical Column
898.28 €
Each
Estimated Shipment: 07-01-2025
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15597520
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Thermo Scientific™
164941
250 mm 0.75 mm 2 μm Analytical Column
1272.57 €
Each
Estimated Shipment: 13-01-2025
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11352013
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Thermo Scientific™
164535
150 mm 0.075 mm 3 μm Trap Column
623.63 €
Pack of 2
In Stock
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15810705
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Thermo Scientific™
164738
500 mm 0.075 mm 2 μm Analytical Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
15820705
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Thermo Scientific™
164739
500 mm 0.075 mm 3 μm Analytical Column This item is currently unavailable or has been discontinued.
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N/A
15567530
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Thermo Scientific™
164942
500 mm 0.075 mm 2 μm Analytical Column
1460.68 €
Each
Estimated Shipment: 16-01-2025
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15572920
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Thermo Scientific™
164939
750 mm 0.075 mm 2 μm Analytical nano Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
15800705
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Thermo Scientific™
164454
50 mm 1 mm 2 μm Analytical Column, NanoViper
1431.16 €
Each
Estimated Shipment: 07-01-2025
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15588090
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Thermo Scientific™
164946
20 mm 75 μm 3 μm Trap Column
611.04 €
Each
In Stock
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12739835
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Thermo Scientific™
164704
150 mm 0.05 mm 2 μm Analytical Column This item is currently unavailable or has been discontinued.
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N/A
15527660
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Thermo Scientific™
164943
150 mm 0.05 mm 2 μm Analytical Column
1116.02 €
Each
Estimated Shipment: 07-01-2025
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11382253
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Thermo Scientific™
164261
250 mm 0.075 mm 3 μm Analytical Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
11342103
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Thermo Scientific™
160321
150 mm 0.075 mm 3 μm Analytical Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
11312263
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Thermo Scientific™
164564
150 mm 0.1 mm 5 μm Trap Column This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
15517460
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Thermo Scientific™
164940
150 mm 0.075 mm 2 μm Analytical Column
1094.57 €
Each
Estimated Shipment: 08-01-2025
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17294434
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Thermo Scientific™
164750
150 mm 0.1 mm 5 μm Trap Column
623.63 €
Pack of 2
In Stock
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Edge
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17204444
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Thermo Scientific™
164751
150 mm 0.3 mm 3 μm Analytical Column, Nanoviper This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
13486836
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Thermo Scientific™
164711
150 mm 1 mm 2 μm Analytical Column
855.95 €
Each
In Stock
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15518080
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Thermo Scientific™
164944
250 mm 0.05 mm 2 μm Column
1272.57 €
Each
Estimated Shipment: 08-01-2025
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Add to basket
 
15588080
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Thermo Scientific™
164945
500 mm 0.05 mm 2 μm Column
1460.57 €
Each
Estimated Shipment: 10-01-2025
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Description

Description

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation.

  • High resolution in protein identification, biomarker discovery, and systems biology
  • Particular loadability allows highest sensitivity in LC/MS
  • Designed for TFA-free LC-MS, minimizing ion-suppression effects
  • Optimized for coupling to ESI/MS and MALDI-MS
  • Highest column-to-column reproducibility
  • Easy-to-use, cutting-edge miniaturized HPLC
  • Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

  • Ease of use, no sleeve for small capillaries
  • Tool-free assembly
  • No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions
  • No experimental failure due to bad connections

Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology.

  • High loading capacity
  • Use for the analysis of low abundant peptides often found in complex proteomics samples
  • Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements
  • Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D.
  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.

Specifications

Specifications

100 Å
L1
C18
Reversed Phase
Videos
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Documents

Documents

Product Certifications

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

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