Antibiotics, Buffers and Supplements
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium
Used to enzymatically release adherent cells from tissue culture plates for passaging.
Gibco™ Bacto™ Tryptone
For use with dehydrated media culture
Thermo Scientific™ Remel™ Soya Peptone Neutralized
A vegetable peptone complying with USP specifications, its high carbohydrate content makes it suitable for many purposes
Corning™ Epidermal Growth Factor (EGF), Mouse Natural (Receptor Grade)
Enhance the proliferation of a wide variety of cell types
G418 Sulfate (White to Off-white Powder), Fisher BioReagents
CAS: 108321-42-2 Molecular Formula: C20H44N4O18S2 Molecular Weight (g/mol): 692.702 InChI Key: UHEPSJJJMTWUCP-TUWLDMFGSA-N PubChem CID: 134129582 IUPAC Name: (2R,3R,4R,5R)-2-[(1S,2R,3R,4S,6R)-4,6-diamino-3-[(2S,3S,4R,5S,6R)-3-amino-4,5-dihydroxy-6-[(1R)-1-hydroxyethyl]oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid SMILES: CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
Gibco™ RPMI 1640
Media with increased levels of vitamins
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
Penicillin/Streptomycin Mixture, Fisher BioReagents
Interferes with synthesis of bacterial cell wall. Inhibits protein synthesis; binds to 30S ribosomal subunit
Corning™ Nu-Serum™ IV Growth Serum Replacements
Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture
Gibco™ Bacto™ Casamino Acids
An animal origin (AO) acid hydrolysis of casein.
Ionomycin (Calcium Salt), Fisher BioReagents
CAS: 56092-82-1 Molecular Formula: C41H70CaO9 Molecular Weight (g/mol): 747.08 InChI Key: WKRWUYKLUMMAKG-QNCSZBBNSA-L PubChem CID: 71311740 IUPAC Name: calcium;(4R,6S,8S,10E,12R,14R,16E,18R,19R,20S,21S)-19,21-dihydroxy-22-[(2S,5S)-5-[(2R,5S)-5-[(1R)-1-hydroxyethyl]-5-methyloxolan-2-yl]-5-methyloxolan-2-yl]-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate SMILES: CC(CCC(=O)[O-])CC(C)CC(C)C(=O)C=C(C(C)CC(C)CC=CC(C)C(C(C)C(CC1CCC(O1)(C)C2CCC(O2)(C)C(C)O)O)O)[O-].[Ca+2]
Corning™ Nu-Serum™ IV Growth Medium Supplement
Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture
Gibco™ Bacto™ Yeast Extract
Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.
Alfa Aesar™ Ampicillin
CAS: 69-53-4 Molecular Formula: C16H19N3O4S Molecular Weight (g/mol): 349.405 MDL Number: MFCD00005175 InChI Key: AVKUERGKIZMTKX-NJBDSQKTSA-N PubChem CID: 6249 ChEBI: CHEBI:28971 IUPAC Name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid SMILES: CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C
Gibco™ Bacto™ Proteose Peptone No. 3
Enzymatic digest of porcine protein
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Corning™ Penicillin-Streptomycin Solution
Effective against Gram-positive and Gram-negative bacteria. Corning™ Penicillin-Streptomycin Solution is a mix of the antibiotics Penicillin (10,000 IU) and Streptomycin (10,000µg/mL) in a 100-fold working concentration.
Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free
Solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Corning™ Matrigel™ Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-Free, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
MP Biomedicals™ Cell Culture Reagents
Used to release adherent cells from culture surfaces. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
Alfa Aesar™ Tetracycline
CAS: 60-54-8 Molecular Formula: C22H24N2O8 Molecular Weight (g/mol): 444.44 MDL Number: MFCD00151232 InChI Key: NWXMGUDVXFXRIG-WESIUVDSSA-N PubChem CID: 54675776 IUPAC Name: (4S,4aS,5aS,6S,12aR)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide SMILES: CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O
Water, for HPLC-MS, Fisher Chemical
Packaging: Amber glass bottle
Gibco™ Bacto™ Peptone
Enzymatic digest of protein