HIC Reagents and Supplies Hydrophobic Interaction Chromatography
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: SDS, Sodium lauryl sulfate PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)
Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.
Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Thermo Scientific™ HA Synthetic Peptide
HA Synthetic peptide 26184 from Thermo Scientific™ specifically detects HA in samples, and it is validated for Control
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.
Thermo Scientific™ Pierce™ Disposable Columns, 5 mL
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Cytiva Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.
Invitrogen™ Magnetic Stand-96
Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories
Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads
Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.
Thermo Scientific™ Detergent Removal Spin Columns & Plates
Rapidly eliminate high concentrations of detergents with minimal sample loss with detergent removal resins. Thermo Scientific HiPPR (High Protein and Peptide Recovery) resin is recommended for protein concentrations up to 100 µg/mL, while Thermo Scientific Pierce resin is suitable for concentrations over 100 µg/mL.
Thermo Scientific™ Pierce™ Centrifuge Columns, 5 mL
Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.
Invitrogen™ DynaMag™-50 Magnet
Optimized for efficient cell isolation using Dynabeads™
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.
Cytiva Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Cytiva Streptavidin HP MultiTrap™ Plates
Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates
Sartorius Vivapure™ LentiSELECT 500 Purification and Concentration Kit
Fast purification of up to 3 x109 viral particles
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
IBA Lifesciences Strep-Tactin™ PE or APC
Fluorophore-conjugated Strep-Tactin™ for cell staining with PE or APC
Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.
Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA
Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction using these cell-free kits.
Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.
Thermo Scientific™ Pierce™ Iminobiotin Agarose
Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.
SDS Solution, 10% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™
CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.38 MDL Number: MFCD00036175 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
Thermo Scientific™ Inclusion Body Solubilization Reagent
Retrieve recombinant proteins that form insoluble aggregates during bacterial overexpression with this reagent optimized for inclusion body solubilization.