Protein Biology Methods

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Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.

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Step 1: Extraction

Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, Lysis reagents, plus additional products and resources.

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Step 2: Clean Up

Once Extracted the sample needs to be cleaned of the impurties that can hinder downstream sample analysis. We offer the full Clean Up workflow, from Dialysis to Desalting and Concentration.

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Step 3: Purification

Purification is another necessary protein biology method and involves separation by protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and Endotoxin removal. For further information, download our resources.

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Step 4: Separation

Solubility, size, charge, and other protein characteristics are used to separate proteins. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.

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Step 5: Protein Detection

Western blotting is frequently used for protein detection. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol.

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Related Resources

Amino Acids Reference Tool

Amino Acids Reference Tool

Learn About Amino Acids

Protein Shows Promise in Treatment of Spinal Cord Injuries

Amino Acids Reference Guide

Download the PDF


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Complete your life sciences chromatography workflow.

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