Western Blotting Reagents, Membranes, Supplies

Merck Millipore Immobilon™-FL PVDF Transfer Membranes

Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

GE Healthcare Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. GE Healthcare Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

Merck Millipore Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use)

Take advantage of a one-step polymer system providing increased sensitivity, time savings and detection simplicity with the Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use).

Invitrogen™ Mini Blot Module

Wet transfer device used exclusively with the Bolt Minigel Tank

GE Healthcare Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.

Invitrogen™ iBlot™ 2 Gel Transfer Device

Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers

Invitrogen™ Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

Thermo Scientific™ PVDF Transfer Membranes

Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.

Thermo Scientific™ Western Blot Roller

A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)

Pre-cut and pre-assembled and ready for protein blotting/transfer

Invitrogen™ CellROX™ Orange Reagent, for oxidative stress detection

Novel fluorogenic probe for measuring oxidative stress in live cells

Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer (Ready-To-Use)

Employ a senstive detection system based on biotin-free, polymer technology.

Invitrogen™ Mini Gel Tank and Blot Module Set

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Thermo Scientific™ Pierce™ Protein-Free (TBS) Blocking Buffer

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

Thermo Scientific™ Blocker™ BLOTTO in TBS

Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, mini

iBlot Transfer Stacks are used to transfer proteins

GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ iBlot™ 2 and iBind™ Western Starter Kit

The iBlot 2 and iBind Western Starter Kit includes the iBlot 2 Gel Transfer Device, the iBind Western Device as well as all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western-processing.

  spinner