Western Blotting Reagents, Membranes, Supplies
Molecular Probes™ Qdot™ 605 Biotin Conjugate Kit
Qdot™ 605 Biotin Conjugate Kit
Merck Millipore Immobilon™-FL PVDF Transfer Membranes
Designed specifically for Western blots and protein sequencing. Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.
GE Healthcare Amersham™ Hyperfilm™ ECL
Use with all blue and green light chemiluminescent systems. GE Healthcare Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.
Merck Millipore Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Thermo Scientific™ Pierce™ CN/DAB Substrate Kit
Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.
Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini
Used to transfer proteins with the iBlot 2 Gel Transfer Device
Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use)
Take advantage of a one-step polymer system providing increased sensitivity, time savings and detection simplicity with the Thermo Scientific™ Lab Vision™ UltraVision™ ONE Detection System: HRP Polymer (Ready-To-Use).
Invitrogen™ Mini Blot Module
Wet transfer device used exclusively with the Bolt Minigel Tank
GE Healthcare Whatman™ Grade GB003 Blotting Paper
Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes.
Invitrogen™ iBlot™ 2 Gel Transfer Device
Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers
Invitrogen™ Shrimp Alkaline Phosphatase (SAP)
Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.
Thermo Scientific™ PVDF Transfer Membranes
Enable high protein and nucleic acid binding capacity with sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane.
Thermo Scientific™ Western Blot Roller
A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
Invitrogen™ Invitrolon™ PVDF/Filter Paper Sandwiches, 0.45 µm, 8.3 x 7.3 cm (for mini gels)
Pre-cut and pre-assembled and ready for protein blotting/transfer
Invitrogen™ CellROX™ Orange Reagent, for oxidative stress detection
Novel fluorogenic probe for measuring oxidative stress in live cells
Thermo Scientific™ Lab Vision™ UltraVision™ LP Detection System: HRP Polymer (Ready-To-Use)
Employ a senstive detection system based on biotin-free, polymer technology.
Invitrogen™ Mini Gel Tank and Blot Module Set
Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels
Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate
Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.
Thermo Scientific™ Pierce™ Protein-Free (TBS) Blocking Buffer
Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.
Thermo Scientific™ Blocker™ BLOTTO in TBS
Block membranes, plates and other samples with this ready-to-use 5% solution of nonfat powdered milk in TBS for Western blot, ELISA and other detection methods.
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, mini
iBlot Transfer Stacks are used to transfer proteins
GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. GE Healthcare Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.
GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Invitrogen™ iBlot™ 2 and iBind™ Western Starter Kit
The iBlot 2 and iBind Western Starter Kit includes the iBlot 2 Gel Transfer Device, the iBind Western Device as well as all of the consumables and reagents necessary to get started using both devices for fast, efficient transfer and western-processing.